Cellulase from  Humicola  spp. | Zendy

Ali Md. Showkat | Zendy; Akhand Anwarul Azim | Zendy; Gomez Paul Fabrian | Zendy; Sarker Rafiqul Islam | Zendy

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Cellulase from Humicola spp.

Author(s) -

Ali Md. Showkat,

Akhand Anwarul Azim,

Gomez Paul Fabrian,

Sarker Rafiqul Islam

Publication year - 1993

Publication title -

journal of basic microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.58

H-Index - 54

eISSN - 1521-4028

pISSN - 0233-111X

DOI - 10.1002/jobm.3620330302

Subject(s) - cellulase , hydrolysis , cellulose , chemistry , ammonium , yeast extract , adsorption , hyacinth , straw , yeast , carbon source , food science , enzyme , fermentation , chromatography , biochemistry , botany , microbiology and biotechnology , biology , organic chemistry , inorganic chemistry

The newly isolated Humicola strain secreted cellulases in the culture medium supplemented with 1% water hyacinth as carbon source. Of the nitrogen sources investigated, ammonium sulphate (0.5%) and yeast extract (4%) produced the highest amount of the enzyme complex. Maximal cellulases were synthesized at pH 6.0 and temperature 35 °C. The culture filtrate from this organism was used to study the saccharification of lignocellulosic wastes and adsorption onto avicel. About 48% saccharification was achieved with rice straw after a period of 24 h hydrolysis. The endoglucanase in the culture filtrate was adsorbed onto avicel to the extent of 13%.

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