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Morpho‐physiologic characteristics of Escherichia coli and Shigella flexneri 2a carrying the htp R15 gene
Author(s) -
Tetz Victor V.,
Rybalchenko Oksana V.,
Savkova Galina A.
Publication year - 1993
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620330210
Subject(s) - shigella flexneri , microbiology and biotechnology , shigella , escherichia coli , morpho , biology , chemistry , gene , genetics , botany
Electron microscopic examination of the morphology of normal Escherichia coli MC4100 and Shigella flexneri cells and their htp R‐defective recombinants has shown the presence in the latter of noncrystalline granules after growth at 37 °C. The defect of the htp R gene leads to some changes in ultrastructure of bacterial colonies and the intensification of the processes of development and aging of colonies as integral structures. These phenomena are manifested by early formation of intercellular contacts and thick surface films as well as the enhanced destruction of microorganisms. The recombinant Shigella strain grown at 30 °C retained its ability to provoke keratoconjunctivitis.