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Selective isolation of fluorescent and nonfluorescent Pseudomonas species from Sohag Governorate (Upper Egypt)
Author(s) -
Shoreit Ahmed A. M.,
Soltan ElSayed
Publication year - 1992
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620320610
Subject(s) - agar , chemistry , isolation (microbiology) , pseudomonas fluorescens , microbiology and biotechnology , pseudomonas , alcaligenes , pseudomonas putida , fluorescence , pseudomonas stutzeri , chromatography , bacteria , botany , biology , biochemistry , genetics , physics , quantum mechanics , enzyme
Three hundred and fourty nine isolates belonging to ten different species of Pseudomonas were isolated and identified from soil and water samples on four selective isolation media: Modified Trypticase soy agar (TSA), ACC‐agar containing ampicillin‐chloramphenicol‐cycloheximide, Nitrate plus organic acid agar and Tryptophan substrate agar. The fluorescent species were P. putida (21.2% of the total Pseudomonas species), P. fluorescens (15.8%), P. aeruginosa (9.7%) and P. aureobaciens (2.6%), while the nonfluorescent species were P. stutzeri (12.4%), P. alcaligenes (11.2%), P. pseudoalcaligenes (9.5%). P. mendocina (8.3%), P. lemoignei (5.7%) and P. ruhlandii (3.4%). Modified TSA and ACC media were efficient for the isolation of both fluorescent and nonfluorescent species, whereas Nitrate and Tryptophan media were more selective for the isolation of nonfluorescent species. The optimum temperature for the isolation of different species ranged between 20 ° and 37 °C.