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Fortuitous resorcinol metabolism by a phloroglucinol‐induced Rhodococcus sp. BPG‐8
Author(s) -
Armstrong S.,
Patel T. R.
Publication year - 1992
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620320602
Subject(s) - phloroglucinol , resorcinol , chemistry , biotransformation , chromatography , thin layer chromatography , phenols , phenol , rhodococcus , metabolism , biochemistry , organic chemistry , enzyme
Abstract A Rhodococcus sp. BPG‐8 isolated from oil‐rich soil in Newfoundland was capable of using phloroglucinol as a sole source of carbon and energy. Culture filtrates of cells grown on resorcinol were extracted and analyzed using gas chromatography‐mass spectra (GC‐MS), thin layer chromatography (TLC), and ultraviolet spectrophotometry. Phloroglucinol induced cell‐free extracts revealed the presence of phloroglucinol hydroxylase that hydroxylated resorcinol. Meta‐cleavage of 1,2,4‐benzenentriol occurs at a slow rate. A tentative biotransformation pathway for resorcinol by Rhodococcus sp. BPG‐8 is discussed.