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Isolation, identification and germination of spores of Thermoactinomyces from Qatari soils (Arabian Gulf)
Author(s) -
Shoreit Ahmed A. M.
Publication year - 1992
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620320210
Subject(s) - spore , germination , soil water , spore germination , botany , chemistry , organic matter , biology , horticulture , ecology
40 isolates of Thermoactinomyces were isolated from Qatari soils and identified as T. vulgaris (26 isolates), T. thalpophilus (8), T. putidus (4) and T. sacchari (2). The total count of this genus increased concomitantly with the increase of organic matter in soil samples. The effect of temperature, pH and composition of medium on the germination of spores of T. vulgaris were examined by measuring the reduction of turbidity of spore suspensions. Optimal germination took place at 55 °C, at a pH range of 7.5 to 8.5 and in phosphate buffer (10 mM) plus L‐alanine, L‐leucine and L‐phenylalanine (1 mM).