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In vivo utilization of N‐(phosphonomethyl)‐anilines and related substances by Pseudomonas spec. GS
Author(s) -
Albrecht Birgit,
Weidhase Rosemarie,
Stock Manfred,
Weidhase Reinhard Albrecht
Publication year - 1991
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620310602
Subject(s) - phosphonate , pseudomonas , toluene , in vivo , chemistry , bond cleavage , amine gas treating , substrate (aquarium) , strain (injury) , biochemistry , stereochemistry , organic chemistry , bacteria , biology , catalysis , ecology , genetics , microbiology and biotechnology , anatomy
Utilization of various phosphonates as source for phosphorus by the glyphosate degrading strain Pseudomonas spec. GS was investigated. Metabolites of phosphonate degradation were characterized indicating the cleavage of the C—P‐bond as primary step of breakdown. The phosphonate N‐(phosphonomethyl)‐4′‐nitroazobenzene‐4‐amine (azophon) was characterized as a suitable substrate for detection of C—P‐bond splitting activity in vivo. Pseudomonas cells permeabilized by toluene treatment were also capable of phosphonate degradation whereas no in vitro activity of a putative C—P‐bond cleaving enzyme was detectable after cell disruption.