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Immunogold labelling of human IFNα2 and an IFNα1/α2 hybrid produced by recombinant Escherichia coli
Author(s) -
Breitling Reinhard,
Wagner Barbara,
Wagner Manfred,
Gase Klaus,
Behnke Detlev
Publication year - 1991
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620310505
Subject(s) - immunogold labelling , periplasmic space , cytoplasm , heterologous , biology , microbiology and biotechnology , subcellular localization , escherichia coli , recombinant dna , gene , chemistry , biochemistry , antibody , genetics
In recombinant Escherichia coli strains the subcellular location of human interferon (IFN)α2 and a hybrid IFNα1/α2 was investigated by immunogold labelling techniques. The gold label was scattered throughout the cytoplasm in cells containing the gene for mature IFNα2 under the control of heterologous staphylokinase sak 42D transcription and translation initiation signals. In contrast, in cells containing in addition the sak 42D signal peptide coding region in front of the IFN gene, the gold label was found mainly near the cell membrane and in the periplasmic space. Inclusion bodies were identified in cells accumulating IFN in the cytoplasm.