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A solid‐phase enzyme immunoassay for the detection of tetanus toxin using human and murine monoclonal antibodies
Author(s) -
Kiessig Stephan T.,
Hentschel Christian,
Jahn Sigbert,
Mehl Michael,
Starke Roland,
Porstmann Thomas
Publication year - 1991
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620310212
Subject(s) - monoclonal antibody , immunoassay , toxoid , toxin , clostridium tetani , antibody , tetanus , chemistry , enzyme , microbiology and biotechnology , biology , virology , biochemistry , immunization , immunology , vaccination
Three human and three murine monoclonal antibodies were tested for their reactivity to tetanus toxin and toxoid and used to establish an enzyme immunoassay specific for tetanus toxin. The dissociation constants of the monoclonal antibodies were between 3.91 × 10 −9 and 8.48 × 10 −12 . Two human monoclonal antibodies recognized conformation determinants on the toxin, whereas the others reacted to the heavy chain. Only a combination of antibodies of the two species allowed the development of an enzyme immunoassay for the detection of tetanus toxin with a lower detection limit of 1.2 μg/l.