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Genetic recombination in Streptomyces michiganensis DSM 40 015 revealed three genes responsible for the formation of melanin
Author(s) -
Held Thomas,
Kutzner Hans J.
Publication year - 1991
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620310211
Subject(s) - auxotrophy , biology , streptomyces , locus (genetics) , gene , genetics , mutant , melanin , microbiology and biotechnology , bacteria
By UV and NTG mutagenesis numerous auxotrophic, antibiotic‐resistant and melanin‐negative mutants were isolated from Streptomyces michiganensis DSM 40 015 which proved to possess a efficient photoreactivation system. Using a mel − test strain with three auxotrophic markers and a antibiotic resistance for crosses with numerous prototrophic mel − strains three classes of mutants ( melA , melB and melC ) could be found. This classification was further supported by a series of crosses. The melC locus seemed to correspond to the melC locus of S. glaucescens which contains the tyrosinase structural gene.