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Host‐controlled modification and restriction as a criterion of evaluating the therapeutical potential of Pseudomonas phage
Author(s) -
Gachechiladze K. K.,
Balardshishvili N. S.,
Adamia R. S.,
Chanishvili T. G.,
Krüger D. H.
Publication year - 1991
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620310206
Subject(s) - lysis , host (biology) , strain (injury) , restriction enzyme , dna , biology , microbiology and biotechnology , genome , pseudomonas aeruginosa , bacteria , genetics , gene , anatomy
The recently isolated phages ØST3 and ØST1 were compared as to their lysis behaviour in about 100 different P. aeruginosa strains. The growth of ØST3 varies greatly in different host strains. We demonstrated one case of “non‐classical”, host‐dependent modification and restriction. Here the capability to adsorb, and consequently to reproduce in a given host strain differs, depending on which modification the phage aquired in its former host. — The DNA‐containing phage ØST1 displays stable lysis properties in the majority of the host strains. This makes ØST1 a candidate for therapeutic phage preparations. One of the reasons for stable lysis properties is the apparent selection against recognition sites of restriction enzymes in its genome.