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Selection of mutant Streptomyces strains with enhanced ability for acid precipitable polymeric lignin production
Author(s) -
Antai S. P.,
Iyo A. H.
Publication year - 1990
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620300905
Subject(s) - spore , mutant , pigment , sugar , incubation , food science , cellulase , chemistry , wild type , lignin , ultraviolet light , streptomyces , biochemistry , biology , microbiology and biotechnology , botany , enzyme , bacteria , organic chemistry , gene , photochemistry , genetics
Streptomyces viridosporus T7A spores were subjected to ultraviolet (UV) light mutagensis. Two mutant strains S. viridosporus T7A‐40 and T7A‐198 were isolated. These strains were grown on 5 g of sugar cane baggase lignocellulose and produced 36.0 mg and 41.4 mg of acid precipitable polymeric lignin (APPL) respectively as compared to 25.1 mg produced by the wild type after 8 weeks incubation at 37 °C. The quantities of APPL produced by the mutants represent 43.7% and 65.3% more APPLs than those produced by the wild type. Apart from the increase in APPL production, the observable effects of the ultraviolet light included; variation in spore pigmentation, induction of a brown diffusible pigment, production of hydrogen sulfide, loss of thermotolerance, lower cellulase activity and loss of inositol utilizing ability.