Premium
A cytoplasmic factor involved in the expression of resistance to C8‐ATC in Saccharomyces cerevisiae
Author(s) -
Misiewicz M. H.,
Kotylak Z.
Publication year - 1990
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620300810
Subject(s) - saccharomyces cerevisiae , cytoplasm , microbiology and biotechnology , biology , chemistry , yeast , genetics
A dozen mutants of Saccharomyces cerevisiae , resistant to C8‐ATC have been characterized. C8‐ATC was previously established as a biologically toxic compound. Frequency of mutants (10 ‐7 ) was typical for spontaneous mutations. One very stable mutant was characterized extensively. The genetical analysis revealed that resistance in this mutant was determined by single‐gene mutation. The rho o cells, obtained by ethidium bromide (EB) mutagenesis of the resistant strain, were completely devoid of resistance. A large percentage of rho ‐ cells, obtained by a moderate EB treatment of resistant cells were still able to express resistance to C8‐ATC. Therefore we hypothesized that, in our particular strain, a cytoplasmic factor is involved in nuclear determination of resistance.