A cytoplasmic factor involved in the expression of resistance to C8‐ATC in  Saccharomyces cerevisiae | Zendy

Misiewicz M. H. | Zendy; Kotylak Z. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

A cytoplasmic factor involved in the expression of resistance to C8‐ATC in Saccharomyces cerevisiae

Author(s) -

Misiewicz M. H.,

Kotylak Z.

Publication year - 1990

Publication title -

journal of basic microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.58

H-Index - 54

eISSN - 1521-4028

pISSN - 0233-111X

DOI - 10.1002/jobm.3620300810

Subject(s) - saccharomyces cerevisiae , cytoplasm , microbiology and biotechnology , biology , chemistry , yeast , genetics

A dozen mutants of Saccharomyces cerevisiae , resistant to C8‐ATC have been characterized. C8‐ATC was previously established as a biologically toxic compound. Frequency of mutants (10 ‐7 ) was typical for spontaneous mutations. One very stable mutant was characterized extensively. The genetical analysis revealed that resistance in this mutant was determined by single‐gene mutation. The rho o cells, obtained by ethidium bromide (EB) mutagenesis of the resistant strain, were completely devoid of resistance. A large percentage of rho ‐ cells, obtained by a moderate EB treatment of resistant cells were still able to express resistance to C8‐ATC. Therefore we hypothesized that, in our particular strain, a cytoplasmic factor is involved in nuclear determination of resistance.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research