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Detection of a 2 μ derivative yeast plasmid with altered properties
Author(s) -
Mink Mátyás,
Stülke Jörg,
Büttner Knut
Publication year - 1990
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620300714
Subject(s) - extrachromosomal dna , plasmid , pbr322 , saccharomyces cerevisiae , biology , insert (composites) , strain (injury) , dna , genetics , homologous chromosome , plasmid preparation , yeast , t dna binary system , microbiology and biotechnology , vector (molecular biology) , recombinant dna , gene , mechanical engineering , anatomy , engineering
The Saccharomyces cerevisiae ( Sacch. cerevisiae ) strain RXII, like many others, harbours plasmid DNAs. and one category of them is homologous to the 2 μ plasmid of yeast. DNA‐DNA hybridization experiments indicated altered structures of this species as regards the number and distribution of the restriction sites. The efforts made to clone either the whole plasmid in pBR328 or its fragments in pBR322 vectors remained unsuccessful, since deleted plasmids were isolated without insert DNA, and even the loss of vector sequences was observed. The data suggest, that the 2 μ derivative plasmid in strain RXII represent an unique category of this extrachromosomal genetic element.