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Substratinduzierte cAMP‐Signale in Wildstämmen und Mutanten von Saccharomyces cerevisiae
Author(s) -
Sachse Ottomar
Publication year - 1990
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620300615
Subject(s) - yeast , saccharomyces cerevisiae , mutant , intracellular , substrate (aquarium) , chemistry , wild type , microbiology and biotechnology , biology , biochemistry , gene , ecology
Addition of glucose or other substrates to starved Saccharomyces cerevisiae cells triggers a cyclic AMP signal which induces the protein phosphorylating cascade. Before the addition of various substrates the wild‐type and mutant yeast strains were arrested at the G 1 phase of the cell division cycle by transferring the cells, grown at 26 °C to 36 °C in a synthetic medium without any substrate. After the temperature shift back to 26 °C different substrates were added and the cAMP levels were measured. The highest cAMP levels were observed immediately after the addition of the substrates. A relationship between the maximum growth rate of the individual strains or mutants at a given substrate and the intracellular cAMP level is discussed.