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Steroid‐1‐dehydrogenases in nocardioform bacteria studied by electrophoresis and immuno blotting techniques
Author(s) -
Kaufmann G.,
Schumann G.,
Wollweber L.,
Hüller E.,
Atrat P.
Publication year - 1990
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620300608
Subject(s) - antiserum , bacteria , enzyme , rhodococcus , blot , dehydrogenase , electrophoresis , reactivity (psychology) , gel electrophoresis , biology , biochemistry , strain (injury) , actinomycetales , microbiology and biotechnology , chemistry , antigen , streptomyces , medicine , genetics , alternative medicine , pathology , anatomy , gene
Fifteen nocardioform bacteria strains, capable of transforming steroid compounds were investigated with regard to their range of inducible steroid‐1‐dehydrogenase (St1DH) 1 activities. The St1DHs of these bacteria were compared due to their immuno reactivity in Western blot experiments with a rabbit antiserum raised against the purified St1DH of Rhodococcus rhodochrous 7030. Four strains exhibited a strong immuno reactivity, irrespective of differences in the electrophoretic mobility of the enzymes. Fivestrains revealed significantly diminished reactivities, and in five strains with a very low St1DH content, no reactivity was found. One strain, designated as Nocardia spec. 7151, exhibited a high, inducible St1DH activity, but no immuno reaction was found. The absence of immuno reactivity is discussed in connection with the considerably diminished electrophoretic mobility of this enzyme.