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Selective and rapid solubilization of the microbial membrane enzyme aldehyde dehydrogenase
Author(s) -
Hommel R.,
Kleber H.P.
Publication year - 1990
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620300418
Subject(s) - aldehyde dehydrogenase , chemistry , aldehyde , enzyme , biochemistry , solubilization , membrane , dehydrogenase , chromatography , catalysis
An improved solubilization procedure for the membrane‐bound quinoprotein aldehyde dehydrogenase from Acetobacter rancens CCM 1774 was established. After the first solubilization of membrane enzymes by Brij 35 which provided important extraction of membrane proteins other than aldehyde dehydrogenase, the application of Trition X‐100 resulted in an almost 20‐fold purification of quinoprotein aldehyde dehydrogenase. The optimal solubilization was closely connected with definite detergent/protein ratios.