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Mutagenesis of Acetobacter methanolicus MB58 with the transposon Tn5
Author(s) -
Dobrowolski P.,
Gründig M. W.
Publication year - 1990
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620300417
Subject(s) - acetobacter , transposon mutagenesis , transposable element , mutagenesis , chemistry , biology , biochemistry , mutation , mutant , fermentation , gene
Transposon mutagenesis was applied to the isolation of mutants of the facultatively methylotrophic Acetobacter methanolicus MB58. The transposon Tn 5 (pSU2011) was transferred from Escherichia coli SM 10 by means of conjugation to Acetobacter methanolicus MB 58. Four out of 1850 stable Km‐resistant transconjugants were identified that were formaldehyde sensitive and failed to grow on methanol.