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Bioenergetic parameters of Acremonium chrysogenum
Author(s) -
Telesnina G. N.,
Bartoshevish Yu. E.,
Krakhmaleva I. N.,
Petyushenko R. M.,
Sazykin Yu. O.,
Navashin S. M.
Publication year - 1990
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620300412
Subject(s) - oxidative phosphorylation , biochemistry , cytochrome c oxidase , penicillium chrysogenum , cephalosporin c , strain (injury) , respiration , bioenergetics , alternative oxidase , biology , cytochrome , mitochondrion , enzyme , chemistry , botany , anatomy , cephalosporin , antibiotics
Bioenergetic parameters of two isogenic strains of Acremonium chrysogenum markedly differing in their capacity for biosynthesis of cephalosporin C were studied. It was shown that in the high productive strain the system of oxidative phosphorylation played the dominant role in the cellular energy supply (the index of the substrate consumption efficiency amounted to 0.82). In both strains terminal oxidation of the reduction equivalents mainly occurred via the respiration chain with cytochrome oxidase as the terminal component. In the low productive strain an alternative cyanide resistant pathway was observed, in addition to the classic cytochrome chain. A procedure suitable for the isolation of stable protoplasts from the mycelium of A. chrysogenum was developed. It was proved to be useful in the isolation of functionally active mitochondria possessing theoretical phosphorylation efficiency and high phosphate acceptor respiration control. It was concluded that there are three oxidative phosphorylation sites in the respiration chain of the high productive strain of A. chrysogenum .