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Interferonsensitivität verschiedener Zellkulturen: Untersuchungen zur Abhängigkeit der relativen antiviralen Aktivität vom Interferon‐Subtyp
Author(s) -
Tonew Marion,
Glück Brigitte,
Ohme Elke,
Wondraczek R. H.
Publication year - 1989
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620290817
Subject(s) - vesicular stomatitis virus , cytopathic effect , interferon , biology , virus , biological activity , cell culture , recombinant dna , microbiology and biotechnology , chemistry , virology , in vitro , biochemistry , genetics , gene
The phenomenon that rHuIFN‐αl(D) displays an apparently higher antiviral activity when assayed on bovine cells as compared to human cell lines was applied to the elucidation of the nature of recombinant HuIFN prepared in our institute. These investigations were carried out by using a microtitre test, which defines biological activity as the IFN concentration leading to 50 % inhibition of the cytopathic effect of vesicular stomatitis virus (VSV). In addition, the ability of IFN to diminish the reproduction of infectious viruses was monitored. The two methods yielded similar results. With bovine cells, antiviral activities of the same order of magnitude were observed, regardless of the interferon types applied, i.e. rHuIFN‐αl. rHuIFN‐α2 and human leukocyte interferon. On human fibroblasts, however, rHuIFN‐αl had an apparently 45 to 165 times lower activity than the other two interferons. On human WISH cells, the differences in apparent activity between the respective IFNs were even greater, with factors of up to 212 fold being observed. Still more distinctive were the effects on murine L 929 cells where an antiviral effect could be confirmed only for rHuIFN‐αl whereas the other two interferons proved completely inactive.