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Expression of β‐glucosidase and endo‐β‐1,4‐glucanase during development of Chaetomium fusisporale and their characterization
Author(s) -
Sandhu D. K.,
Puri R.
Publication year - 1989
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620290811
Subject(s) - extracellular , isozyme , chaetomium , enzyme , biochemistry , cytosol , chemistry , glucanase , cellulase , carboxymethyl cellulose , biology , sodium , food science , penicillium , organic chemistry
In the present study, the production of β‐glucosidase and endoglucanase and the pattern of their corresponding polymorphic forms occurring in the extracellular and cytosolic filtrates of Chaetomium fusisporale (ATCC 64772) has been studied. When C. fusisporale was grown on carboxymethyl cellulose (CMC) as the sole carbon source, the extracellular fractions contained most of these enzymes. The distribution of the enzymes varied with the age of the culture. By the use of electrophoretic techniques the presence of two forms of β‐glucosidase, i.e. β‐GLU II and β‐GLU III was detected in extracellular and cytosolic filtrates at early stages of growth, while at later stages two more isozymes of β‐glucosidase, i.e. β‐GLU I and β‐GLU IV, only appeared in the cytosolic filtrates. Similary, endoglucases isozymes EG I and EG II were present in both fractions at early stages of growth but at later stages, with the production of perithecia, EG III appeared. Thus, EG III, β‐GLU I and β‐GLU IV may indicate that they are correlated with the development of the fungus. In order to differentiate further between the multiple molecular forms of these enzymes two of them have been characterized with respect to temperature, pH, ethylenediamine tetra‐acetic acid (EDTA) and metal ions.