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Beladung von Polystyrol Festphasen mit mikrobiellen Erregern und deren Polysaccharid‐Antigenen zum Screening monoklonaler Antikörper
Author(s) -
Mehl M.,
Starke R.,
Urban Anette,
Hoffmann A.,
Kiessig St.,
Schmidt G.
Publication year - 1989
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620290612
Subject(s) - microbiology and biotechnology , monoclonal antibody , antigen , candida albicans , chemistry , antibody , biology , immunology
Before screening monoclonal antibodies using enzyme immuno assays, it is necessary to prove that the coating of the solid phases occurs efficiently. The adhesion of whole cells of group B streptococci, Escherichia coli K1, Haemophilus influenzae type b and Candida albicans as well as B‐ Streptococcus group antigen, H. influenzae type b capsular antigen, and C. albicans cell wall antigen to polystyrene solid phases was studied and detected by the following methods:. Peroxidase competition procedure, scintillations measurement of 32 phosphate‐labelled germs, and reactions with monoclonal antibodies.