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Chemische Charakterisierung des Escherichia coli K 1 Kapselpolysaccharides
Author(s) -
Mehl Michael,
Starke Roland,
Kissig Bettina,
Grunow Roland,
Jahn Siegbert,
Schmidt Gerhard,
von Baehr Ruediger
Publication year - 1989
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620290408
Subject(s) - escherichia coli , antigen , chemistry , monoclonal antibody , neuraminic acid , microbiology and biotechnology , serology , biochemistry , escherichia , polysaccharide , biology , antibody , glycoprotein , gene , immunology , genetics
The exact chemical characterization of the prepared Escherichia coli K 1 capsule polysaccharide is necessary and a prerequisite for using this antigen as a screening antigen in the production of monoclonal anti‐K 1‐antibodies. The K 1‐antigen, prepared by phenol‐water‐extraction, was analyzed by protein, RNA, and neuraminic acid determination. An addition, the antigen was subjected to elementary analysis, infrared‐ and 13 C‐NMR‐spectroscopy, and gelchromatography. After testing the serological specificity, the K 1‐antigen was identified as a high molecular polyneuraminic acid.