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An efficient method for producing and purifying gramme quantities of T‐2 toxin
Author(s) -
Lepom Peter,
Baath Helga
Publication year - 1989
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620290405
Subject(s) - toxin , chromatography , chemistry , reagent , thin layer chromatography , silica gel , column chromatography , fusarium , biology , biochemistry , organic chemistry , botany
A simple procedure was developed for the laboratory production and purification of gramme quantities of T‐2 toxin. After growing at 22 °C on rice for two weeks, Fusarium sporotrichioides T‐21 produced average T‐2 toxin concentrations of 1309 mg/kg and as much as 1000 mg of crystalline product were recovered. The method described avoids long‐term, low temperature incubations. Crude culture extracts were rapidly purified in two steps by column chromatography on silica gel. The purity of the crystallized product was verified by thin‐layer chromatography using various spray reagents for detection.