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Einsatz der Fermenter‐Dialyse‐Kultur zur Züchtung kapseltragender Bakterien mit klinischer Bedeutung
Author(s) -
Starke Roland,
Mehl Michael,
Möckel Claudia,
Grunow Roland,
Montag Thomas,
von Baehr Rüdiger,
Schmidt Gerhard
Publication year - 1989
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620290316
Subject(s) - industrial fermentation , microbiology and biotechnology , bacteria , monoclonal antibody , chemistry , biology , chromatography , food science , antibody , immunology , fermentation , genetics
For mass cultivation of group B streptococci (B‐III and B‐variant streptococci), a fermenter dialysis culture technique is described and compared to conventional shaking culture and fermenter batch culture techniques. The influence of two kinds of cultivation media on the bacterial yield is demonstrated. The growth rate of the bacteria and the yield of the microbes is higher in modified POPE medium than that observed with Nährbouillon I. The type‐specific polysaccharide of B‐III‐streptococci prepared by phenol‐water extraction followed by gelchromatography can be used as a screening antigen for the production of monoclonal antibodies against B‐III‐streptococci.