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Methodische Untersuchungen zur Interferon‐Induktion unter in vitro ‐Bedingungen
Author(s) -
Glück Brigitte,
Stelzner Axel
Publication year - 1989
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620290103
Subject(s) - in vitro , inducer , fibroblast , interferon , priming (agriculture) , chemistry , cell culture , cell , microbiology and biotechnology , biology , biochemistry , immunology , genetics , gene , botany , germination
The procedures generally used for the in vitro induction of interferon exploit fibroblast or leucocyte culture suspensions. Several factors and conditions influencing the capacity for IFN induction were studied, with the following results. In contrast to previous findings, optimum IFN induction was obtained at 37 °C, rather than 30 °C, when human fibroblasts were induced with 100 μg/ml of Poly (IC). There was a direct correlation between the dose of the inducing agent applied and the amount of IFN produced. Also, the cells were excellently superinducible with metabolic inhibitors like actinomycin D and cycloheximine. In addition, priming procedures enhanced IFN induction in human fibroblasts. Comparing different cell types with respect to their ability to be induced for IFN production, we found that different cell types show great differences in their inducer sensitivity. Finally, the response of different cell culture systems was highly variable when induced with different compounds.