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Nachweis einer direkten Isomerisierung von Mannose zu Fructose und ihre Bedeutung für den Mannosestoffwechsel von Pseudomonas syringae pv. phaseolicola
Author(s) -
Sauerstein J.,
Ramm M.,
Schubert K.,
Reuter G.
Publication year - 1988
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620280928
Subject(s) - isomerase , chemistry , enzyme , mannose , pseudomonas syringae , fructose , pseudomonas , pseudomonadales , bacteria , biochemistry , biology , gene , genetics
The phytopathogenic pseudomonad Pseudomonas syringae pv. phaseolicola possesses an isomerase which converts mannose to fructose. The synthesis of this enzyme is constitutive. The K M value for mannose is 0.02 M. Fe 2 + , Mg 2 + and Mn 2 + ions stimulate the enzyme activity, whereas Cu 2 + and Zn 2 + iones are inhibitors. Since the enzymes mannokinase and phosphomannose isomerase could not be detected, the isomerase and an inducible fructokinase must have a function in the metabolism of mannose by this bacterium.