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Microscopic structure of ineffective alfalfa nodules formed by auxotrophic mutants of Rhizobium meliloti
Author(s) -
Małek Wanda
Publication year - 1988
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620280925
Subject(s) - rhizobium , root nodule , nodule (geology) , mutant , auxotrophy , cytoplasm , biology , arginine , strain (injury) , bacteria , inoculation , botany , microbiology and biotechnology , symbiosis , biochemistry , horticulture , genetics , anatomy , amino acid , gene , paleontology
The inoculation of lucerne seedlings with mutants of Rhizobium meliloti (Arg − ) requiring arginine resulted in the formation of ineffective nodules. In these nodules bacteria were not released from the infection threads into the nodule cytoplasm. When arginine was provided as a nitrogen source, the nodules became anatomically similar to those induced by the wild type strain and they fixed nitrogen. Effective nodules were induced also by prototrophic forms of revertants and transductants. The infected cells in the central tissue of the effective nodule were enlarged, whereas uninfected ones of noneffective nodules of Arg − mutants remained small and contained small nuclei and many large starch granules.