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Mutants of Rhizobium meliloti defective in nodulation
Author(s) -
Małek Wanda
Publication year - 1988
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620280924
Subject(s) - mutant , nod , biology , plasmid , rhizobium , root hair , rhizobium leguminosarum , rhizobiaceae , microbiology and biotechnology , ecori , strain (injury) , gene , genetics , symbiosis , bacteria , anatomy
Using nitrosoguanidine (NTG) two nodulation defective (Nod − ), auxotrophic mutants of the Rhizobium meliloti strain L5.30 have been isolated. In their Sym plasmid, pRmeL5.30b, a large deletion has been demonstrated. Both mutants were adsorbed into root hairs of lucerne, but were unable to induce root hair curlings. The nodulation of lucerne was restored to both hair‐curling (Hac − ) mutants by the introduction of the Sym plasmid pJB5JI of R. leguminosarum and by plasmid pEK25, carrying a 3.6 kb AvaI/EcoRI fragment of “common” nod genes of R. meliloti 41. This led to the conclusion that in these Nod − deletion mutants nod mutations were concerned with “common” nod genes.