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Characterization of a thermosensitive mutant of Streptomyces hygroscopicus defective in both DNA and RNA syntheses
Author(s) -
Süss F.,
Frunder B.,
Klaus S.,
Noack D.
Publication year - 1988
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620280816
Subject(s) - streptomyces hygroscopicus , mutant , dna , rna , dna synthesis , escherichia coli , biology , temperature sensitive mutant , elongation , biochemistry , streptomyces , microbiology and biotechnology , genetics , bacteria , gene , materials science , ultimate tensile strength , metallurgy
A stable temperature sensitive mutant of Streptomyces hygroscopicus JA6599 defective in both DNA and RNA syntheses is described. The mutant ts35 is characterized by an immediate stop of DNA synthesis and continued protein synthesis after transfer to restrictive temperature. The reinitiation of DNA synthesis begins immediately after a return to the permissive temperature. This kinetics of macromolecular synthesis at restrictive temperature appears to share similarities with a defect in the DNA elongation process, as described for Escherichia coli (C ARL 1970, H ANNA and C ARL 1975). The simultaneous stop of both DNA and RNA syntheses may be caused by an additional mutational event affecting also the RNA synthesis. The data were discussed with respect to similar results in E. coli .