Premium
Taxonomic studies of Acinetobacter species based on the electrophoretic analysis of enzymes
Author(s) -
Nishimura Yukimasa,
Kanzaki Hisanaga,
Iizuka Hiroshi
Publication year - 1988
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620280605
Subject(s) - acinetobacter , acinetobacter calcoaceticus , strain (injury) , electrophoresis , enzyme , polyacrylamide gel electrophoresis , cluster (spacecraft) , biology , gel electrophoresis , microbiology and biotechnology , bacteria , biochemistry , genetics , anatomy , computer science , programming language
Thirty six strains of Acinetobacter , 22 strains from the culture collections and 14 isolates from soil and cotton samples, were examined for the presence of twelve enzymes using polyacrylamide gel electrophoresis. A numerical analysis was carried out on the basis of similarity values obtained from the electrophoretic mobilities of the enzymes. The strains used were divided into four clusters (Z‐1, Z‐2, Z‐3 and Z‐4), and cluster Z‐1 was further divided into three subclusters (Z‐1a, Z‐1b and Z‐1c). The type strains of Acinetobacter calcoaceticus IAM 12087 (ATCC 23055) and Acinetobacter Iwoffii ATCC 15309 were found to belong to subcluster Z‐1a and cluster Z‐4, respectively. The radiation‐resistant strain FO‐1, a new type of acinetobacters, was included in cluster Z‐3. Superoxide dismutase showed clearly distinguishable mobilities among four clusters, and was concluded to be useful in grouping acinetobacters.