A gene‐cloning system for  Kluyveromyces lactis  and isolation of a chromosomal gene required for killer toxin production | Zendy

Chen X. J. | Zendy; WésolowskiLouvel M. | Zendy; TanguyRougeau C. | Zendy; Bianchi M. M. | Zendy; Fabiani L. | Zendy; Saliola M. | Zendy; Falcone C. | Zendy; Frontali L. | Zendy; Fukuhara H. | Zendy

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A gene‐cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production

Author(s) -

Chen X. J.,

WésolowskiLouvel M.,

TanguyRougeau C.,

Bianchi M. M.,

Fabiani L.,

Saliola M.,

Falcone C.,

Frontali L.,

Fukuhara H.

Publication year - 1988

Publication title -

journal of basic microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.58

H-Index - 54

eISSN - 1521-4028

pISSN - 0233-111X

DOI - 10.1002/jobm.3620280402

Subject(s) - kluyveromyces lactis , plasmid , complementation , transformation (genetics) , gene , cloning (programming) , biology , kluyveromyces , structural gene , genetics , saccharomyces cerevisiae , microbiology and biotechnology , escherichia coli , phenotype , computer science , programming language

A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis . The principle is essentially equivalent to that of the 2 μ/ Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1‐based DNA bank of K. lactis , the KEX1 gene involved in the killer system was isolated by complementation.

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