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A gene‐cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production
Author(s) -
Chen X. J.,
WésolowskiLouvel M.,
TanguyRougeau C.,
Bianchi M. M.,
Fabiani L.,
Saliola M.,
Falcone C.,
Frontali L.,
Fukuhara H.
Publication year - 1988
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620280402
Subject(s) - kluyveromyces lactis , plasmid , complementation , transformation (genetics) , gene , cloning (programming) , biology , kluyveromyces , structural gene , genetics , saccharomyces cerevisiae , microbiology and biotechnology , escherichia coli , phenotype , computer science , programming language
A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis . The principle is essentially equivalent to that of the 2 μ/ Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1‐based DNA bank of K. lactis , the KEX1 gene involved in the killer system was isolated by complementation.