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Strain selection in Kluyveromyces marxianus var. lactis for galactose production
Author(s) -
Marin M.Hélène,
Boze Hélène,
Moulin G.,
Galzy P.
Publication year - 1987
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620270909
Subject(s) - galactokinase , galactitol , lactose , galactose , kluyveromyces lactis , biochemistry , kluyveromyces , kluyveromyces marxianus , lactose permease , strain (injury) , mutant , beta galactosidase , biology , chemistry , permease , yeast , saccharomyces cerevisiae , gene , gene expression , escherichia coli , anatomy
After treatment with ethy1‐methane sulfonate (EMS) mutants of Kluyveromyces marxianus var, lactis altered in galactose metabolism were obtained which were further imporoved by genetic recombination. Six types of mutants were detected. In batch culture strains with double mutations — either in galactokinase and uridyltransferase, or in galactokinase and epimerase‐ and which also had a galactose permease transformed lactose to galactose and galactitol. Strains with the same double mutations but no galactose permease completely hydrolyzed lactose to galactose and galactitol under continuous culture.