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Effect of growth temperature on the externalization and localization of α‐amylase in Bacillus stearothermophilus
Author(s) -
Bezbaruah R. L.,
Pillai K. R.,
Gogoi B. K.,
Singh H. D.,
Baruah J. N.
Publication year - 1987
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620270905
Subject(s) - amylase , extracellular , protoplasm , externalization , intracellular , exponential growth , enzyme , chemistry , biochemistry , biology , biophysics , cytoplasm , psychology , mathematical analysis , mathematics , psychoanalysis
The synthesis and localization of α‐amylase were studied in Bacillus stearothermophilus AN002 at 40, 45, 50 and 55 °C and also during early exponential, late exponential and stationary phases of growth. The extracellular α‐amylase synthesis was highes at 50 °C and at stationary growth phase. Accumulation of intracellular α‐amylase was observed in cells grown at 40 °C and was distributed in all the cell fractions. At 50 and 55 °C the cell did not have α‐amylase in the protoplasm or protoplamic membrane. The synthesis and externalization of extracellular enzyme are two processes and the externalization is controlled by growth temperature and cell age.