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Nucleoid segregation pattern during branching in Streptomyces granaticolor mycelia
Author(s) -
Kretschmer Sigrid
Publication year - 1987
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620270409
Subject(s) - hypha , nucleoid , germ tube , mycelium , biology , branching (polymer chemistry) , spore , elongation , sporogenesis , microbiology and biotechnology , botany , chemistry , genetics , gene , escherichia coli , organic chemistry , materials science , ultimate tensile strength , metallurgy
A survey of GIEMSA‐stained S. granaticolor mycelia grown in liquid medium revealed that branches developed only in subapical older regions of main hyphae for which large nucleoids were characteristic. The branches themselves contained only small nucleoids, as did the apical (about 30 μm long) region of the main hyphae. From the distribution pattern of the nucleoids at nascent branching areas it was concluded that during branch outgrowth one large nucleoid segregation into two small ones, both of which in most cases entered the branch. In order to determine in vivo the number of genome equivalents provided from main hyphae for a single branch, the kinetics of outgrowth of branches was measured and compared to that of germ tubes. The analysis of series of micrographs revealed that at the beginning of outgrowth, a branch received two genome equivalents from the main hypha while a germ tube got only one (from the spore). The subsequently expressed exponential elongation of branches as well as germ tubes ruled out that further nucleoids were supplied thereafter.