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Nachweis einer NAD abhängigen Gluconat‐6‐phosphat‐Dehydrogenase in Pseudomonas syringae pv. phaseolicola
Author(s) -
Sauerstein J.,
Jacob J.,
Reuter G.
Publication year - 1987
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620270314
Subject(s) - dehydrogenase , pentose phosphate pathway , nad+ kinase , biochemistry , chemistry , enzyme , phosphogluconate dehydrogenase , microbiology and biotechnology , glucose 6 phosphate dehydrogenase , biology , glycolysis
Crude extracts from cells of Pseudomanas syringae pv. Phaseolicola , a fluorescent pseudomonad, when grown on glucose contain a NAD‐linked 6‐phosphogluconate dehydrogenase. The reaction of the enzyme, which produces 14 CO 2 from 1‐ 14 ‐C‐6‐phosphogluconate, is not inhibited by NaF, a potent inhibitor of the ENTER‐DOUDOROFF (ED) pathway enzyme 6‐phosphogluconate dehydratase. In the presence of phosphate or arsenate ions the NAD‐linked glyceraldehyde‐3‐phosphate dehydrogenase reacts with glyceraldehyde‐3‐phosphate which, in the ED pathway, is produced from 6‐phosphogluconate and overlaps the 6‐phosphogluconate dehydrogenase reaction. Only a small proportion of glucose is metabolized via the 6‐phosphogluconate dehydrogenase/oxidative pentose phosphate pathway.