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Hemagglutinating activity in phytopathogenic bacteria surface compounds
Author(s) -
Serra M. Teresa,
Castresana M. Carmen,
Tejerina Genoveva
Publication year - 1987
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620270306
Subject(s) - erwinia , xanthomonas campestris , bacteria , microbiology and biotechnology , biology , pseudomonas , xanthomonas , ralstonia solanacearum , agglutination (biology) , pathogenic bacteria , pathogen , antibody , genetics , immunology
Extracellular components of plant pathogenic bacteria were obtained from their culture medium as well as from the whole cells by using NaCl 1 M, pH 6.0; 20% sucrose dissolved in 0.03 M Tris buffer, pH 8.0; or 0.05 M Na 2 EDTA. All the extracts from Erwinia carotovora subsp. carotovora, Xanthomonas campestris pv. campestris, Pseudomonas syrinagae pv. phaseolicola, Xanthomonas campestris pv. phaseoli, Pseudomonas solanacearum , and Erwinia carotovora subsp. atroseptica , were assayed for hemagglutinating activity on sheep, rabbit and chicken red blood cells (RBCs). The only active extracts were those obtained by NaCl treatment. They agglutinated sheep and rabbit erythrocytes. Extracts from E. carotovora subsp. atrose ptica gave rise to the high agglutination titer on rabbit RBCs. These extracts had the lowest polysaccharide/protein ratio. E. carotovora subsp. carotovora extracts showed only a low titer (18.5 units). The agglutinating activity present in NaCl extracts of the bacteria tested was inhibited by different carbohydrates to various extent. Extracts from E. carotovora subsp. atroseptica appeared to be the most sensitive ones while those of E. carotovora subsp. carotovora least sensitive to the presence of sugar. It is suggested that hemagglutinins observed in plant pathogenic bacteria and those in plant host are similar and that both may, in some way, be involved in the plant‐parasite relationship.