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Effect of nourseothricin (streptothricin) on the outer membrane of sensitive and resistant Escherichia coli strains
Author(s) -
Seltmann Guntram,
Wolter ErnstJürgen
Publication year - 1987
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620270305
Subject(s) - escherichia coli , microbiology and biotechnology , chemistry , bacterial outer membrane , biology , biochemistry , gene
Nourseothricin (streptothricin) causes disturbances (perforations) in the outer membrane of sensitive E. coli strains allowing lysozyme and deoxycholate, but not the periplasmic alkaline phosphatase to penetrate. EDTA slightly increases, but Mg ++ ions slightly decrease this effect. The cell walls of three from four nourseothricin‐resistant strains do not become permeable under these conditions, but remain sensitive against TRIS/EDTA. Nourseothricin is supposed to pass the outer membrane of sensitive bacteria via some kind of “self‐promoting” pathway. This way can (but need not) be blocked in resistant strains.