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RNA polymerase binding sites on a plasmid R6K derivative with increased copy number
Author(s) -
Nešvera Jan,
Klaus Siegfried,
Štokrová Jitka,
Hochmannová Jitka
Publication year - 1987
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620270109
Subject(s) - plasmid , microbiology and biotechnology , polymerase , dna polymerase i , rna polymerase , transcription (linguistics) , dna , escherichia coli , biology , dna polymerase , chemistry , rna , genetics , gene , reverse transcriptase , linguistics , philosophy
The specific binding of Escherichia coli RNA polymerase molecules to the DNA of plasmid pNH602, a deletion derivative of R6K having an increased copy number, was detected by electron microscopy. Seven strong RNApol binding sites were found on pNH602 DNA linearized with Bam HI or Eco RI restriction endonuclease. All of these specific sites occur in genetically defined regions of the pNH602 molecule. Two of them correspond with the recently reported transcription initiation sites within a region essential for plasmid R6K replication.