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Differences in enzyme activities of Lipomyces starkeyi between cells accumulating lipid and proliferating cells
Author(s) -
Naganuma T.,
Uzuka Y.,
Tanaka K.,
Iizuka H.
Publication year - 1987
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620270106
Subject(s) - biochemistry , dehydrogenase , enzyme , citric acid cycle , lipid droplet , atp citrate lyase , biology , lactate dehydrogenase , glycolysis , yeast , cell growth , citrate synthase , chemistry
Differences in intracellular enzyme activities between cells accumulating lipid and proliferating cells were studied with Lipomyces starkeyi , a fat‐producing yeast. When the cells were removed from a medium for lipid accumulation to a medium for cell proliferation, activities of several enzymes on the glycolytic pathway, pentosephosphate cycle, glycerol 3‐phosphate pathway, tricarboxylic acid cycle and fatty‐acid biosynthesis pathway changed. In the proliferating cells, activities of glycerol‐3‐phosphate dehydrogenase (GDH) and ATP citrate lyase were very low, as compared to those in the cells accumulating lipids. Activities of these two enzymes and 6‐phosphogluconate dehydrogenase, particularly GDH, decreased with the addition of zinc ion to the cell‐free extract of the cells accumulating lipid.