Premium
Synthesis of poly ( A ) + ‐containing RNA during growth in Escherichia coli relA + and relA − strains
Author(s) -
Hanschke R.,
Hecker M.
Publication year - 1986
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620260603
Subject(s) - rna , transcription (linguistics) , polyadenylation , protein biosynthesis , messenger rna , stringent response , biology , biochemistry , post transcriptional modification , amino acid , ribosomal rna , microbiology and biotechnology , escherichia coli , chemistry , rna dependent rna polymerase , gene , philosophy , linguistics
There are very different results on the synthesis of poly ( A ) + ‐containing RNA in bacteria. We, therefore, studied the influence of growth and amino acid starvation on the synthesis of poly(A) + ‐RNA in a relA + and relA − strains of E. coli . Only the relA + strains is able to respond to an amino acid limitation by production of ppGpp which causes a strong reduction of stable RNA transcription. During growth we observed significant alterations of the percentage of [ 3 H]‐uridine labelled total RNA which bound to poly(U)‐sepharose (% poly(A)‐RNA). It was mainly influenced by drastic changes of the synthesis of non‐polyadenylated stable RNA (rRNA, tRNA) during growth and, therefore, it did not reflect the actual synthesis of polyadenylated mRNA. An amino acid starvation induced in the relA + strain a stronger and more rapid reduction of the transcription of non‐polyadenylated RNA as well as of poly ( A ) + ‐RNA than in the relA − strain which did not produce ppGpp under these conditions. Therefore, we conclude that ppGpp inhibited not only the synthesis of the non‐polyadenylated stable RNA but also that of poly ( A ) + ‐containing mRNA, although the latter was apparently less affected.