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Effect of temperature on growth and protein turnover in Bacillus megaterium
Author(s) -
Strnadová M.,
Prasad R.,
Kučerová H.,
Chaloupka J.
Publication year - 1986
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620260511
Subject(s) - bacillus megaterium , arrhenius plot , protein turnover , composition (language) , protein degradation , degradation (telecommunications) , chemistry , food science , biochemistry , growth rate , biology , botany , zoology , protein biosynthesis , activation energy , bacteria , organic chemistry , telecommunications , genetics , linguistics , philosophy , computer science , geometry , mathematics
Abstract The Arrhenius plot of the specific growth rate μ of an asporogenic mutant of Bacillus megaterium , growing in the range of 17–37 °C followed a straight line, whose slope was affected by the composition of the medium. The maximal μ was found at 40° C in all media tested and decreased rapidly above 42° C. The portion of protein degraded also followed a straight line but reached its maximal value at 45–47° C. Its slope was affected substantially by the composition of the medium; the mixture of amino acids suppressed protein degradation at temperature lower than 37° C. The extent of the short‐lived protein fraction, degraded with the half‐time of about 1 h, was mostly affected by both — the temperature and the medium. The amount of proteins degraded per one biomass doubling was almost constant up to 37° C but was affected by the composition of the medium. At higher temperatures it increased rapidly and reached values of 20–40% of pulselabelled proteins degraded per one cell cycle. The degradation of ethionine‐containing proteins was similarly dependent on the temperature but the extent of proteins degraded was increased.