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Catabolism of androst‐4‐ene‐3,17‐dione by mutant strains of Nacardia restricta
Author(s) -
Miclo A.,
Germain P.,
Lefebvre G.
Publication year - 1986
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620260206
Subject(s) - catabolism , mutant , chemistry , biochemistry , ene reaction , enzyme , dehydrogenase , stereochemistry , biosynthesis , metabolite , steroid , inducer , gene , hormone
Different mutants of the catabolism of androst‐4‐ene‐3,17‐dione were produced in Nocardia restricta by action of γ‐rays and nitrosoguanidine. These selected mutants (acetate + ‐androst‐4‐ene‐3,17‐dione − ) are hexanoate − . Their behaviour is identical to the mutants selected as hexanoate − acetate + which are themselves incapable of growing on androst‐4‐ene‐3,17‐dione. These mutants degrade androst‐4‐ene‐3,17 dione to methylperhydroindane dione propionic acid in presence of acetate with a molar yield of about 50%. The study of the biosynthesis of the 1,2‐steroid‐dehydrogenase in the mutant strains shows that a metabolite of MEPHIP is an inducer of the biosynthesis of this enzyme. On the other hand, the behaviour of these mutant strains shows an inductible β‐oxidation mechanism which takes place in the catabolism of methyl perhydroindane dione propionic acid. Another isolated mutant strain degrades androst‐4‐ene‐3,17‐dione with an transient accumulation of 3‐hydroxy‐9,10‐secoandrosta‐1,3,5(10)‐triene‐9,17‐dione.