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Expression of the streptokinase gene from Streptococcus equisimilis in Bacillus subtilis
Author(s) -
Klessen Christian,
Malke Horst
Publication year - 1986
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620260203
Subject(s) - bacillus subtilis , streptokinase , plasmid , gene , transcription (linguistics) , microbiology and biotechnology , recombinant dna , biology , bacteria , chemistry , biochemistry , genetics , myocardial infarction , psychology , linguistics , philosophy , psychiatry
The previously cloned and sequenced streptokinase gene ( skc ) from Streptococcus equisimilis H46A was inserted into plasmid vectors capable of replication in Bacillus subtilis . The skc gene was expressed by use of its own transcription and translation signals which appeared to meet the stringent requirements of B. subtilis for efficient foreign gene expression. The secreted streptokinase activity began to decline toward the end of the exponential growth phase suggesting that B. subtilis exoproteases hydrolyzed and inactivated the foreign protein.