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Rapid isolation of bacterial proteinases by column chromatography on sawdust
Author(s) -
Šafařík I.
Publication year - 1985
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620251023
Subject(s) - chromatography , elution , chemistry , sawdust , column chromatography , isolation (microbiology) , sodium , enzyme , biochemistry , microbiology and biotechnology , biology , organic chemistry
Proteolytic enzymes produced by some strains of Bacillus sp. were isolated by column chromatography on sawdust. Ballast proteins were eluted with water while the adsorbed proteinases were eluted with 1 m sodium chloride solution. The specific activities of proteinases increased 54‐ to 70‐fold after chromatography.