Premium
Metabolism of methanol by yeast and SCP production
Author(s) -
Jwanny Etidal W.,
Rashad Mona M.
Publication year - 1985
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620251009
Subject(s) - methanol , chemistry , biochemistry , yeast , campesterol , metabolism , food science , amino acid , glutathione , yield (engineering) , composition (language) , chromatography , organic chemistry , cholesterol , sterol , enzyme , materials science , linguistics , philosophy , metallurgy
The utilization of methanol by Candida lipolytica was studied with respect to the biomass, protein, lipid and other metabolites contents. Supplementation of the methanol medium with certain redox agents such as 2,6‐dichlorophenol indophenol, reduced glutathione and methyl red, showed a stimulation of both growth and protein production of C. lipolytica. At certain growth conditions the cellular yield of C. lipolytica was 0.3 g of cells/g of methanol and the protein of the biomass was 41.0%. The amino acid composition of the protein especially of the essential ones was comparable to FAO standards. Thin‐layer chromatography of the lipids indicated a dominance of total glycerides. Analyses of the acylated unsaponified fraction by gas liquid chromatography showed that campesterol was the major component.