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pH‐linked control of energy charge in Acetobacter methanolicus sp. MB 70
Author(s) -
Loffhagen N.,
Babel W.
Publication year - 1985
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620250906
Subject(s) - energy charge , extracellular , adenylate kinase , chemistry , incubation , intracellular ph , biophysics , intracellular , drop (telecommunication) , biochemistry , biology , enzyme , telecommunications , computer science
The influence of the extracellular pH on the adenylate system of the acidophilic Acetobacter methanolicus sp. MB 70 was investigated. The ATP level was maintained over a pH range from 3.0 to 6.0. An increased endogenous respiration was observed after a raise of the pH from 4.0 upwards. Above pH 6.0 the level of ATP decreased and the level of ADP and even more that of AMP increased. This resulted in a drop of the energy charge from 0.78 to values below 0.2. At pH 4.0 the level of ATP and AMP decreased only after prolonged incubation without an external energy source but the energy charge remained constant at about 0.7. In the range of external pH values where the energy charge is maintained the intracellular pH value is expected to be stable. The investigation of the reasons for the displacement of this range to low extracellular pH values may help to more profoundly understand the phenomenon of acidophily.