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Gene expression at each stage of the life cycle of spore‐forming bacteria: Different sensitivity of transcription to antibiotics which act on DNA gyrase
Author(s) -
Matsuda M.,
Kameyama T.
Publication year - 1985
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620250708
Subject(s) - dna gyrase , novobiocin , biology , nalidixic acid , bacillus subtilis , spore germination , transcription (linguistics) , dna synthesis , mutant , microbiology and biotechnology , dna , germination , rna , gene expression , spore , gene , biochemistry , bacteria , escherichia coli , antibiotics , botany , genetics , linguistics , philosophy
Effects of antibiotics acting on DNA gyrase, novobiocin and nalidixic acid on RNA synthesis during germination, vegetative growth and sporulation of Bacillus subtilis were examined. These drugs were relatively ineffective in inhibiting RNA synthesis of phase Gm 1 (5–15 min) during germination but effective in those of Gm 2 (15–40 min) and Gm 3 (40–60 min) during germination (Matsttda and Kameyama 1980). No distinguishable inhibitory effects of RNA synthesis in B. subtilis Novritt mutant could be detected by novobiocin. Bna synthesis of Gm 2 and Gm 3 of this mutant was inhibited by nalidixic acid. When novobiocin was added to exponential vegetative cell or sporulating cell culture at T 0 and T 1 stage, the rate of Bna synthesis was inhibited by 80% for 5 min following addition of the drug. However, Bna synthesis after T 2 of sporulation became resistant toward novobiocin, as was the case at an early stage of germination. RNA profiles from transcripts synthesized on administration of Nov suggested that the suppression of the synthesis of 23S and 16S rRNA is relatively greater than 4 to 5S RNA at the middle stage of germination and at vegetative growth stage in the presence of NOV. Our present data suggest that DNA gyrase is involved in the regulation of gene transcription during middle and late phases of germination, vegetative growth and T 0 and T 1 of sporulation. The transcription during early phase of germination and sporulation after T 2 may proceed independently of this enzyme.