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Inducible nature of the steroid 11‐hydroxylases in spores of Cunninghamella elegans (Lendner)
Author(s) -
Jaworski A.,
Sedlaczek L.,
Dlugoński J.,
Zajaczkowska Ewa
Publication year - 1985
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620250703
Subject(s) - spore , sporogenesis , hydroxylation , cycloheximide , transformation (genetics) , enzyme , inducer , chemistry , biology , biochemistry , microbiology and biotechnology , protein biosynthesis , gene
Spores harvested from steroid‐free media (non‐induced during sporogenesis), and used for cortexolone hydroxylation in the presence of cycloheximide to prevent enzyme synthesis during transformation produced cortexolone derivatives at a low rate: 4.85 ± 2.37 mg products per g dry weight per 6 hours. Spores induced during sporogenesis and transformation formed the hydroxylation products at the rate 40.25 ± 6.25 mg/g d.wt./6 h, whereas spores induced only once — on the stage of sporogenesis or during transformation — produced them at the rate 15.28 ± 4.80 – 18.85 ± 4.48 mg/g·6 h. The effectiveness of the hydroxylase induction during steroid transformation could be increased if spores pretreated with diluted KOH were used. This agent was shown to loosen the rigid structure of the spore envelopes, and to increase their permeability for the enzyme inducer. The similar could be shown with spores preincubated in a growth medium for 1–3 hours. Transformation rates of 51.34 mg/g·6 h and 68.82 mg/g·6 h were attained.