Premium
Enzym‐Inhibitoren aus Actinomyceten II. Inhibitoren saurer Proteinasen
Author(s) -
Passarge Margrit,
Fleck W. F.
Publication year - 1985
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620250615
Subject(s) - fermentation , streptomyces , chemistry , biochemistry , agar , actinomycetales , microbiology and biotechnology , biology , bacteria , genetics
In the course of a screening program directed toward the isolation and evaluation of inhibitors of carboxyl (acid) proteinases, we have found 0.39% of the investigated strains of microorganisms as producers of pepsin inhibitors. In more than 1543 strains which were isolated from various geographical areas and deposited with the IMET Culture Collection, Jena‐GDR, and which belong to different genera of actinomycetes such as Streptomyces, Actinomadura, Actinoplanes, Micromonospora and non‐identified strains, we have found 6 Streptomyces strains only. Surprisingly, the screening efficiency of the used casein agar spot test and the fibrin‐blue‐technique was very low. As compared with the inhibitor activity of pepstatin the yield of the fermentation broth of 3 wild type strains ranged from 6 to 27 μg/ml. A significant influence of the composition of culture fluids on the inhibitor yield was observed. Supplements by crude glutamine or mixtures of amino acids in culture fluids increased the yield from 3 to 15 times as compared with that of fermentation broth without supplements. After NMG‐mutagenic treatment and use of familiar selection procedures 3.1% inhibitor‐blocked mutants were detected among 480 strains selected.