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Hybridization of yeasts by protoplast fusion: Attempts to polyploidize Pichia guilliermondii
Author(s) -
Klinner U.,
Böttcher F.
Publication year - 1985
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620250403
Subject(s) - protoplast , biology , hybrid , yeast , mitosis , dna , chromosome , aneuploidy , genetics , pichia , cell fusion , microbiology and biotechnology , botany , recombinant dna , gene , pichia pastoris , cell culture
The DNA content of fusion hybrids of Pichia guilliermondii was not further increased by renewed fusions. Occasionally, the stabilized hybrids only contained approximately the DNA content of the corresponding wild‐type strain. Investigation of the early events after fusion revealed spontaneous mitotic segregation with a high frequency. The benomyl‐induced mitotic segregation pattern of the hybrid clones was not uniform. In some clones certain markers did not segregate any longer. This probably indicates the loss of the respective chromosome. The inability of P. guilliermondii fusion products to form stable hybrids with the expected high DNA content may be attributed to either the aneuploidy of the fusion partners or a specific mitotic apparatus of this yeast.